![]() However, β-TCPs show certain limitations such as poor bonding properties and restricting cell attachment and connection to surrounding tissues caused by a comparably low surface reactivity resulting in negative effects on osteogenic properties. Porous β-TCPs are osteoconductive, safe in clinical use and closely mimic the anorganic portion of bone. TCP appears in different polymorphs and is mostly used as β-TCP in orthopedic applications. Ĭurrently, the most frequently used synthetic bone grafting materials are calcium phosphates (CaPs) such as tricalcium phosphates (TCP Ca 3(PO 4) 2). ![]() The development of synthetic bone grafts is an especially attractive and important field, since synthetic materials can not only be produced in large quantities but can also be tailored to meet specific needs in their anticipated field of application. Since the current clinical gold standard-iliac crest bone grafting-can only provide a certain amount of bone grafting material and is potentially followed by donor site complications, the search for appropriate biomaterials that can either reduce or replace the use of autologous tissues is in the spotlight of current orthopedic research. Since most of the stimulatory effects induced by BG particles took place initially, particles exhibiting another surface-area-to-volume ratio should be considered in order to provide long-lasting stimulation.īone defect treatment belongs not only to the most challenging fields in orthopedic surgery, but is also one of the most relevant clinical procedures in modern medicine and will be of increasing importance due to the demographic development. Especially the early stages of osteogenic differentiation were significantly improved in the presence of BG particles, resulting in earlier maturation of the MSC towards osteoblasts. It was demonstrated that BG particles decrease cell proliferation but do not have a negative impact on cell vitality. Cell vitality, proliferation, and osteogenic differentiation were monitored by quantitative gene expression analysis, determination of alkaline phosphatase activity, PrestoBlue cell viability assay, dsDNA quantification, and a fluorescence-microscopy-based live/dead-assay. ![]() For that purpose, Vitoss and Vitoss BA scaffolds were seeded with human mesenchymal stem cells (MSC) and underwent osteogenic differentiation in-vitro for up to 42 days. This study aims to analyze the influence of BG particles in Vitoss BA (20 wt% BG particles with a size of 90–150 μm) on osteogenic properties, cell vitality and cell proliferation in direct comparison to Vitoss by evaluation of the underlying cellular mechanisms. These limitations might be overcome by addition of 45S5 bioactive glass (BG) particles. β-TCPs exhibit osteoconductive properties, however material-initiated stimulation of osteogenic differentiation is limited. ©2023 H&E Equipment Services, the amount of autologous bone for the treatment of bone defects is limited and harvesting might cause complications, synthetic bone substitutes such as the popular β-tricalcium phosphate (β-TCP) based Vitoss have been developed as an alternative grafting material. Articulating Narrow Boom 40-45' w/Jib HybridĪrticulating Narrow Boom 46-59' w/Jib Hybrid
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